Drug Induced Liver Injury prediction

Drug Induced Liver Injury (DILI) prediction

Cryopreserved Human Hepatocytes in vitro (in suspension and plateable) constitute a valuable tool for DILI predictions in humans:

Prediction Model

We constructed a database from 424 drugs eliciting hepatotoxicity in human and built a prediction model In silico performance of prediction model. We performed in vitro assessments (Oxidative Stress, Mitochondrial Injury, Steatosis, Necrosis, Cholestasis) using Cryopreserved Human Hepatocytes. Based on known compounds, the model predicts DILI with 90% accuracy. (Muller et al., 2015)

Role of Inflammation

More recently we found that human plateable CryoHeps represent a valuable tool for mechanistic evaluation of DILI, i.e. the potential role of inflammatory stress: addition of lipopolysaccharide (LPS, see figure below), and addition of non-parenchymal cells (Richert et al., 2016).