Services

KaLy-Cell
offers contract services with hepatocytes from animal and human origin

Contact Lysiane Richert, PhD - Cell phone : +336 07 04 70 00 - Email : l.richert@kaly-cell.com

Toxicity tests on hepatocytes in suspension or cultured Methodology

Cell viability

- MTT

- Caspase 3

Oxidative-stress related toxicity

- Reduced Glutathione

- Glutathione peroxidase

- Catalase

- Superoxide dismutase

Richert et al., TIV 2002
Binda et al. TIV 2003
Martin et al., J Nutrition 2003
Martin et al. J Am College of Nutrition 2006
Metabolism-related tests on hepatocytes in suspension or cultured Methodology

Determination of metabolic stability, intrinsic clearance, metabolic clearance and profile

Microsome incubations

Hepatocyte incubations (eventually analysis)

- Monolayers or Suspensions
   Freshly isolated
   Cryopreserved

Blanchard et al., Current Drug Metabol, 2004
Blanchard et al., Xenobiotica 2005
Jouin et al., EJPB 2006
Blanchard et al., J Pharm Pharmacol 2006
Pekthong et al., Ethnopharmacology 2008
Genomic analysis

Hepatocyte incubations (eventually analysis)

- Monolayers or Suspensions
   Freshly isolated
   Cryopreserved

Richert et al., DMD 2006
Martin et al., Magnesium Research 2007
Induction tests with drugs on cultured hepatocytes for evaluating
the enzyme-inducing potential Methodology

Determination of inducibility

- Monolayers
   Freshly isolated
   Cryopreserved

- mRNA analysis

- CYP and UGT analysis
   Cell microsomes
   Cell monolayers

- Screening or Regulatory Purposes (Final Report)

Viollon-Abadie et al., TIV 2000
Richert et al., TAP 2003
Richert et al., DMD 2008

KaLy-Cell - Bioparc - Boulevard Sébastien Brant - 67400 Illkirch - France - Tel : +333 88 10 88 30 - Fax : +333 88 43 56 71