When Cryopreserved Human Hepatocytes (CryoHeps) attach to culture dishes, they are useful to evaluate short-term and long-term repeat-toxicity as well as enzyme-induction potential of drug candidates (details).

 

KaLy-Cell checks lots of Cryopreserved Human Hepatocytes to ascertain their ability to attach to collagen substratum. Each lot that meets this performance criteria is thereafter characterized for basal Drug Metabolizing Enzyme (DME) activities.  This allows the study of DME-dependent donor-donor variability assessment in metabolism and toxicity of drug candidate (details).

 

Available Cryopreserved Human Plateable Hepatocytes:

 Category Assured Minimum Yeld per vial 
2-4 2-4 x106 viable cells
4-6  4-6 x106 viable cells 
6-8 6-8 x106 viable cells 
8-10  8-10 x106 viable cells 
10-12 10-12 x106 viable cells 

 

 

 

DME characterization of Cryopreserved Human Plateable Hepatocytes

CYP1A2

Phenacetin O-dealkylation

CYP2B6

Bupropion hydroxylation

CYP2C8

Paclitaxel hydroxylation

CYP2C9

Diclofenac 4′-hydroxylation

CYP2D6

Bufuralol hydroxylation

CYP3A4/5

Testosterone 6β-hydroxylation

CYP3A4/5

Midazolam 1′-hydroxylation

 

General UGT

7-hydroxycoumarin-glucuronide

UGT1A1

Beta-estradiol-3-glucuronide

UGT1A3

Chenodeoxycholic-acid-24-glucuronide

UGT1A4

Trifluopirazine-N-glucuronide

UGT1A6

4-hydroxyindole-glucuronide

UGT1A9

Propofol-O-glucuronide

UGT2B7

Naloxone-glucuronide

General SULT

7-hydroxycoumarin-sulfate

 

Other characterizations of Cryopreserved Human Plateable Hepatocytes

Specific lots are further characterized for:

  • concentration-dependent response to prototypical inducers. These are useful to evaluate enzyme-induction potential of drug candidates, (Alexandre et al., 2012).

  • long-term culture in a sandwich configuration. These are useful for long-term repeat-exposure toxicity and metabolism studies (Parmentier et al., 2017).    

  • Suitable for drug-Induced-Cholestasis-Index determination (Chatterjee te al., 2014 and Oorts et al., 2016).

  • B-Clear transporter-qualified (details).

 

For more informations about our Cryopreserved Human Plateable Hepatocytes batches characteristics and inventory feel free to contact us.

Each product is accompanied by a datasheet containing donor informations (age, ethnicity, gender, cause of death or pathology, medication and infectious disease status), post-thaw yield and viability and characterization data.     

Included with each order of Cryopreserved Human Plateable Hepatocytes:

  • Our recommended thawing protocol. 
  • If you order for the first time, we will organize a training session to teach you the protocol at your convenience.
  • Our recommended Plating protocol 
  • Sample preparation worksheet
  • MSDS

Storage and handling of Cryopreserved Human Plateable Hepatocytes

Cryopreserved Human Plateable Hepatocytes should be stored in liquid nitrogen or at -150°C.

 

Caution

biohazardKaLy-Cell has the ability by the French Ministry of Research (AC-2014-2155) to prepare and store products from human origin for research purposes (CSP articles L 1243-3 and L 1243-4), and the ability by the French Ministry of Research to export human samples (CSP article L 1245-5). KaLy-Cell also has the agreement of the French Agency of Biomedicine (CSP article L 1232-3). KaLy-Cell only accepts tissue from donors who test negative for HIV 1 and 2, HTLV, and Hepatitis B and C. However, as a precaution, all human-derived samples should be regarded as a potential biohazard and should be stored, handled and discarded accordingly. Cellular and subcellular fractions are intended for in vitro use only

For more information

Contact us

Register for our
HepatAlert notifications
for fresh or cryoplateable hepatocytes.

Request a quote!

Contact us

We look forward to
helping you find the best
solution for your
compound development
program.